Sign on
ADS Classic is now deprecated. It will be completely retired in October 2019. This page will automatically redirect to the new ADS interface at that point.

SAO/NASA ADS General Science Abstract Service


· Find Similar Abstracts (with default settings below)
· Electronic Refereed Journal Article (HTML)
· Full Refereed Journal Article (PDF/Postscript)
· Citations to the Article (13) (Citation History)
· Refereed Citations to the Article
· Reads History
·
· Translate This Page
Title:
Transgenic mice susceptible to poliovirus.
Authors:
Koike, Satoshi; Taya, Choji; Kurata, Takeshi; Abe, Shinobu; Ise, Iku; Yonekawa, Hiromichi; Nomoto, Akio
Affiliation:
AA(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AB(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AC(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AD(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AE(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AF(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.), AG(Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.)
Publication:
Proceedings of the National Academy of Sciences of the United States of America, Volume 88, Issue 3, 1991, pp.951-955
Publication Date:
02/1991
Origin:
JSTOR; PNAS
DOI:
10.1073/pnas.88.3.951
Bibliographic Code:
1991PNAS...88..951K

Abstract

Poliovirus-sensitive transgenic mice were produced by introducing the human gene encoding cellular receptors for poliovirus into the mouse genome. Expression of the receptor mRNAs in tissues of the transgenic mice was analyzed by using RNA blot hybridization and the polymerase chain reaction. The human gene is expressed in many tissues of the transgenic mice just as in tissues of humans. The transgenic mice are susceptible to all three poliovirus serotypes, and the mice inoculated with poliovirus show clinical symptoms similar to those observed in humans and monkeys. Rabbit antipoliovirus serum detects the antigens mainly in motor neurons in the anterior horn of the spinal cord and in nerve cells in the medulla oblongata and pons of the paralyzed transgenic mice. Therefore, cell types sensitive to poliovirus in the central nervous system of the transgenic mice appear to be identical to those of humans and monkeys. Furthermore, many more doses of oral poliovirus vaccine strains than of the virulent strains are required to cause paralysis in the transgenic mice. This may reflect the observation that the virulent strain multiplies more efficiently in the central nervous system than the attenuated strain. Thus, the transgenic mice may become an excellent new animal model to study molecular mechanisms of pathogenesis of poliovirus and to assess oral poliovirus vaccines.
Bibtex entry for this abstract   Preferred format for this abstract (see Preferences)


Find Similar Abstracts:

Use: Authors
Title
Abstract Text
Return: Query Results Return    items starting with number
Query Form
Database: Astronomy
Physics
arXiv e-prints